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  • CAPS Activity in Priming Vesicle Exocytosis Requires CK2

    CAPS phosphoryla tion by CK2 was constitutive but the elevation of Ca2 in syn aptosomes increased CAPS Ser 5 and 6 dephosphorylation which terminates CAPS activity These results identify a func tionally important N terminal phosphorylation site that regu lates CAPS activity in priming

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  • The Vesicle Priming Factor CAPS Functions as a Homodimer

    Neurotransmitters and peptide hormones are secreted by regulated vesicle exocytosis CAPS also known as CADPS is a 145 kDa cytosolic and peripheral membrane protein required for vesicle docking and priming steps that precede Ca 2 triggered vesicle exocytosis CAPS binds phosphatidylinositol 4 5 bisphosphate PI 4 5 P 2 and SNARE proteins and is proposed to promote

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  • CAPS 1 and CAPS 2 Are Essential Synaptic Vesicle Priming

    Before transmitter filled synaptic vesicles can fuse with the plasma membrane upon stimulation they have to be primed to fusion competence The regulation of this priming process controls the strength and plasticity of synaptic transmission between neurons which in turn determines many complex brain functions We show that CAPS 1 and CAPS 2 are essential components of the synaptic vesicle

  • Secretory Vesicle Priming by CAPS Is Independent of Its

    Cell Reports Report Secretory Vesicle Priming by CAPS Is Independent of Its SNARE Binding MUN Domain Cuc Quynh Nguyen Truong 1 4 Dennis Nestvogel 2 3 4 Olga Ratai 1 Claudia Schirra 1 David R Stevens 1 Nils Brose 3 JeongSeop Rhee 2 3 and Jens Rettig1 1Institute of Physiology Saarland University Building 59 66421 Homburg/Saar Germany 2Neurophysiology Group Max Planck

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  • CAPS activity in priming vesicle exocytosis requires CK2

    CAPS Ca 2 dependent activator protein for secretion functions in priming Ca 2 dependent vesicle exocytosis but the regulation of CAPS activity has not been characterized Here we show that phosphorylation by protein kinase CK2 is required for CAPS activity Dephosphorylation eliminated CAPS activity in reconstituting Ca 2 dependent vesicle exocytosis in permeable and intact

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    Pump priming works in Singapore Download PDF Published 12 December 1996 Pump priming works in Singapore Lee Kheng Cheok 1

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    Fig 3 Self priming pump Centrifugal pump with two casing chambers prior to commissioning Two phase mixture is pumped until the suction line has been evacuated and the fluid level has been pushed into the front suction intake chamber by atmospheric pressure During normal pumping operation this pump works like an ordinary centrifugal pump

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